Part:BBa_K4601002

Jaws

This part is the E. coli codon optimized version of Jaws halorhodopsin.

Usage and Biology

Jaws is a halorhodopsin derived from Haloarcula (Halobacterium) salinarum (strain Shark) and is naturally red-shifted [1]. Compared to the wild-type HR (Halo57) it contains two mutations K200R and W214F that do not modify the absorption properties, but increase the photocurrent generated upon light irradiation.

Halorhodopsins are chloride inward pumps activated by light [1].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601202.

References

[1] Chuong AS, Miri ML, Busskamp V, Matthews GAC, Acker LC, Sørensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nature Neuroscience (2014) 17: 1123–1129.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 581
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 816
  
• 1000
  COMPATIBLE WITH RFC[1000]